期刊：Rice
主题：通气促进水稻根部Cd滞留
标题：Aeration Increases Cadmium (Cd) Retention by Enhancing Iron Plaque Formation and Regulating Pectin Synthesis in the Roots of Rice (Oryza sativa) Seedlings
影响因子：3.513
检测指标：O₂、Cd²⁺流速
检测部位：距离根尖200微米、500微米，木质部
O₂、Cd²⁺流实验处理方法：
3周龄水稻幼苗，50uM CdCl2及充空气（每小时30分钟）处理14天
O₂、Cd²⁺流实验测试液成份：
0.01mM CdCl2,0.1mM KCl, 0.1mM CaCl2 and 0.3mM MES , pH 5.4
作者：浙江理工大学熊杰、李沪波

英文摘要

Aeration and water management increasingrhizosphere oxygen amount significantly promote rice (Oryza sativa) growth andyield, but the effect of root aeration on cadmium (Cd) toxicity andaccumulation in rice seedlings under hydroponic culture remains unclear.

Results showed that aeration promoted riceseedling growth and alleviated Cd toxicity. Transverse section discovered thatCd accelerated root mature and senescence while aeration delayed the mature andsenescence of roots. Non-invasive Micro-test Technology (NMT) showed thataeration increased net O2 and Cd2+ influxes on the surface of roots whiledecreased net Cd2+ influx in xylem. Perls blue staining showed that aerationand Cd treatments increased iron plaque formation on the surface of roots.Results of metal concentration analysis showed that besides increasing Cdretention in iron plaque, aeration also increasing Cd retention in the cellwall of rice roots. Cell wall component analysis showed that aeration not onlyincreased pectin content but also decreased pectin methylesterification degree(PMD) by increasing pectin methylesterase (PME) activity.

All of these results indicate that aerationnot only delays root mature and senescence but also increases Cd retention in rootsby enhancing iron plaque formation and regulating pectin synthesis in the rootsof rice seedlings.

 

中文摘要（谷歌机翻）

曝气和水分管理增加根际氧气量显着促进了水稻（Oryza sativa）的生长和产量，但根系曝气对水培过程中水稻幼苗镉（Cd）毒性和积累的影响仍不清楚。

结果表明，曝气促进了水稻幼苗生长，减轻了Cd的毒性。横切面发现Cd加速根成熟和衰老，而通气延迟了根的成熟和衰老。非损伤微测技术（NMT）显示，通气增加了根表面的净O2和Cd2+流入，同时降低了木质部中的净Cd2+流入。Perls蓝染色显示通气和Cd处理增加了根表面的铁斑块形成。金属浓度分析结果表明，除了增加铁斑块中Cd的保留外，还可以增加水稻根细胞壁中Cd的保留。细胞壁成分分析表明，通气不仅可以增加果胶含量，还可以通过增加果胶甲酯酶（PME）活性来降低果胶甲酯化程度（PMD）。

所有这些结果表明，通气不仅可以延缓根系成熟和衰老，还可以通过增强水稻幼苗根系中铁斑块形成和调节果胶合成来增加根中Cd的保留。

结果表明：在伸长和分生区域检测到明显的O2净流速而不是木质部。相反，在木质部中检测到密集的Cd2 +净流速，在分生区检测到较少的Cd2 +净流速，在伸长区检测到最小的Cd2 +净流速。此外，分生组织区的Cd2 +净流速随持续时间逐渐减少。在有或没有Cd胁迫的情况下，曝气明显增加了水稻根伸长和分生区的O2净流速.Cd处理在最初几分钟增加了水稻根伸长和分生区的O2净流速，然后增加O2净流速恢复到对照逐渐变平。对于Cd2 +净流速，通气明显增加了伸长区的Cd2 +净流速，同时降低了木质部的Cd2 +净流速。虽然Cd2 +净流速随持续时间逐渐减少，但通气也略微增加了分生区的Cd2 +净流速。

 

文章链接：https://thericejournal.springeropen.com/articles/10.1186/s12284-019-0291-0