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中关村NMT联盟“一带一路”全国测试服务网络测试服务信息

4月8日,某研究所将NMT技术应用于钙信号研究,测试样品为小麦,测试指标为Ca2+,在旭月研究院完成实验。| 5月9号,某研究院将NMT技术应用于逆境生理领域,测试样品为黄瓜幼苗,测试指标为NO3-、NH4+,在旭月研究院完成实验。| 6月2号,某研究院将NMT技术应用于逆境胁迫领域,测试样品为棉花苗,测试指标为Ca2+、H+、K+、Na+、IAA,在旭月研究院完成实验。| 6月5号,某研究院将NMT技术应用于植物逆境领域,测试样品为苜蓿,测试指标为K+,在旭月研究院完成实验。| 6月9号,某研究所将NMT技术应用于水稻逆境领域,测试样品为水稻,测试指标为Na+、Ca2+,在中国科学院植物研究所完成实验。| 6月11号,某研究院将NMT技术应用于植物抗逆领域,测试样品为酵母细胞,测试指标为IAA,在旭月研究院完成实验。| 6月16号,某高校将NMT技术应用于昆虫研究,测试样品为昆虫,测试指标为Ca2+、K+,在旭月研究院完成实验。| 6月19号,某研究院将NMT技术应用于植物抗逆领域,测试样品为拟南芥,测试指标为Ca2+,在旭月研究院完成实验。|

NMT历史上的今天丨Plant Science 野大麦对盐胁迫时间的Na+、K+协同响应文章发表


NMT历史上的今天

2012年8月19日,厦门大学郑海雷、陈娟利用NMT在PLoS ONE上发表了标题为Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity的研究成果。

 

  • 期刊:PLoS ONE
  • 主题:NO调控红树耐盐机制
  • 标题:Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity
  • 影响因子:3.730
  • 检测指标:Na+,K+流速
  • 检测部位:红树根(距离根尖20mm)
  • Na+,K+流实验处理方法:红树幼苗,400mM NaCl/100mM SNP/400mM NaCl+100mM SNP处理15天,100 mM阿米洛利/500 mM钒酸盐/200 mM cPTIO瞬时处理
  • Na+,K+流实验测试液成份:0.1 mM KCl, 0.1 mM CaCl2, 0.1 mM MgCl2,0.5 mM NaCl, 0.2 mM Na2SO4, 0.3 mM MES, pH 6.0
  • 通讯作者:厦门大学郑海雷、陈娟

英文摘要

It is well known that nitric oxide (NO) enhances salt tolerance of glycophytes. However, the effect of NO on modulating ionic balance in halophytes is not very clear. This study focuses on the role of NO in mediating K+/Na+ balance in a mangrove species, Kandelia obovata Sheue, Liu and Yong.

We first analyzed the effects of sodium nitroprusside (SNP), an NO donor, on ion content and ion flux in the roots of K. obovata under high salinity. The results showed that 100 μM SNP significantly increased K+ content and Na+ efflux, but decreased Na+ content and K+ efflux. These effects of NO were reversed by specific NO synthesis inhibitor and scavenger, which confirmed the role of NO in retaining K+ and reducing Na+ in K. obovata roots.

Using western-blot analysis, we found that NO increased the protein expression of plasma membrane (PM) H+-ATPase and vacuolar Na+/H+ antiporter, which were crucial proteins for ionic balance. To further clarify the molecular mechanism of NO-modulated K+/Na+ balance, partial cDNA fragments of inward-rectifying K+ channel, PM Na+/H+ antiporter, PM H+-ATPase, vacuolar Na+/H+ antiporter and vacuolar H+-ATPase subunit c were isolated. Results of quantitative real-time PCR showed that NO increased the relative expression levels of these genes, while this increase was blocked by NO synthesis inhibitors and scavenger.

Above results indicate that NO greatly contribute to K+/Na+ balance in high salinity-treated K. obovata roots, by activating AKT1-type K+ channel and Na+/H+ antiporter, which are the critical components in K+/Na+ transport system.

 

中文摘要(谷歌机翻)

众所周知,一氧化氮(NO)增强了糖类植物的耐盐性。然而,NO对调节盐生植物中离子平衡的影响尚不十分清楚。本研究的重点是NO在红树植物Kandelia obovata Sheue,Liu和Yong中介导K+/Na+平衡的作用。

我们首先分析了NO供体硝普钠(SNP)对高盐度下K. obovata根部离子含量和离子通量的影响。结果表明,100μMSNP显着增加K+含量和Na+外排,但降低Na+含量和K+外排。特异性NO合成抑制剂和清除剂逆转了NO的这些作用,证实了NO在保留K+和减少K. obovata根中Na+中的作用。

使用western-blot分析,我们发现NO增加了质膜(PM)H+-ATPase和液泡Na+/H+逆向转运蛋白的蛋白表达,这是离子平衡的关键蛋白。为了进一步阐明NO调节的K+/Na+平衡的分子机制,分离了内向整流K+通道,PM Na+/H+逆向转运蛋白,PMH+-ATP酶,液泡Na+/H+逆向转运蛋白和液泡H+-ATPase亚基c的部分cDNA片段。定量实时PCR的结果显示NO增加了这些基因的相对表达水平,而这种增加被NO合成抑制剂和清除剂阻断。

以上结果表明NO通过激活AKT1型K+通道和Na+/H+反向转运蛋白,在高盐度处理的K. obovata根中对K+/Na+平衡有很大贡献,这是K+/Na+转运系统的关键组分。

Effects of NaCl and SNP on K+ and Na+ fluxes in K. obovata roots. After 15 days of exposure to NaCl (400 mM), SNP (100 mM) and NaCl (400 mM)+SNP (100 mM), the net K+ (A) and Na+ (C) fluxes from the mature root zone (20 mm from the tip) of K. obovata seedlings. The positive value in the figures represents the net influx and negative value represents the net efflux. Each point represents the mean value of four individual roots and bars represent the SE of the mean. The mean fluxes of K+ (B) and Na+ (D) within the measuring periods are shown. Columns labeled with different letters indicates significantly different at P,0.05.

文章链接:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0071543