中关村NMT联盟“一带一路”全国测试服务网络测试服务信息

4月8日,某研究所将NMT技术应用于钙信号研究,测试样品为小麦,测试指标为Ca2+,在旭月研究院完成实验。| 5月9号,某研究院将NMT技术应用于逆境生理领域,测试样品为黄瓜幼苗,测试指标为NO3-、NH4+,在旭月研究院完成实验。| 6月2号,某研究院将NMT技术应用于逆境胁迫领域,测试样品为棉花苗,测试指标为Ca2+、H+、K+、Na+、IAA,在旭月研究院完成实验。| 6月5号,某研究院将NMT技术应用于植物逆境领域,测试样品为苜蓿,测试指标为K+,在旭月研究院完成实验。| 6月9号,某研究所将NMT技术应用于水稻逆境领域,测试样品为水稻,测试指标为Na+、Ca2+,在中国科学院植物研究所完成实验。| 6月11号,某研究院将NMT技术应用于植物抗逆领域,测试样品为酵母细胞,测试指标为IAA,在旭月研究院完成实验。| 6月16号,某高校将NMT技术应用于昆虫研究,测试样品为昆虫,测试指标为Ca2+、K+,在旭月研究院完成实验。| 6月19号,某研究院将NMT技术应用于植物抗逆领域,测试样品为拟南芥,测试指标为Ca2+,在旭月研究院完成实验。|

NMT历史上的今天丨Mol Plant 胞外ATP通过异源G蛋白和ROS促进气孔开放


NMT历史上的今天

2011年12月02日,河北师范大学尚忠林、郝立华、王伟霞、陈忱利用NMT在Molecular Plant 上发表了标题为Extracellular ATP promotes stomatal opening of Arabidopsis thaliana through heterotrimeric G protein a subunit and reactive oxygen species的研究成果。

 

  • 期刊:Molecular Plant
  • 主题:胞外ATP通过异源G蛋白和ROS促进气孔开放
  • 标题:Extracellular ATP promotes stomatal opening of Arabidopsis thaliana through heterotrimeric G protein a subunit and reactive oxygen species
  • 影响因子:4.296
  • 检测指标:Ca2+、H+流速
  • 通讯作者:河北师范大学尚忠林、郝立华、王伟霞、陈忱

英文摘要

In recent years, adenosine tri-phosphate (ATP) has been reported to exist in apoplasts of plant cells as a signal molecule. Extracellular ATP (eATP) plays important roles in plant growth, development, and stress tolerance.

Here, extracellular ATP was found to promote stomatal opening of Arabidopsis thaliana in light and darkness. ADP, GTP, and weakly hydrolyzable ATP analogs (ATPγS, Bz-ATP, and 2meATP) showed similar effects, whereas AMP and adenosine did not affect stomatal movement. Apyrase inhibited stomatal opening. ATP-promoted stomatal opening was blocked by an NADPH oxidase inhibitor (diphenylene iodonium) or deoxidizer (dithiothreitol), and was impaired in null mutant of NADPH oxidase (atrbohD/F).

Added ATP triggered ROS generation in guard cells via NADPH oxidase. ATP also induced Ca2+ influx and H+ efflux in guard cells. In atrbohD/F, ATP-induced ion flux was strongly suppressed. In null mutants of the heterotrimeric G protein α subunit, ATP-promoted stomatal opening, cytoplasmic ROS generation, Ca2+ influx, and H+ efflux were all suppressed.

These results indicated that eATP-promoted stomatal opening possibly involves the heterotrimeric G protein, ROS, cytosolic Ca2+, and plasma membrane H+-ATPase.

中文摘要(谷歌机翻)

近年来,已经报道三磷酸腺苷(ATP)作为信号分子存在于植物细胞的质外体中。细胞外ATP(eATP)在植物生长,发育和胁迫耐受性中起重要作用。

在这里,发现细胞外ATP在光与暗中促进拟南芥气孔的开放。ADP,GTP和弱水解性ATP类似物(ATPγS,Bz-ATP和2meATP)显示出相似的作用,而AMP和腺苷并不影响气孔运动。磷酸酶抑制气孔开放。ATP促进的气孔开放被NADPH氧化酶抑制剂(二亚苯基碘鎓)或脱氧剂(二硫苏糖醇)阻止,并且在NADPH氧化酶的无效突变体(atrbohD / F)中受损。

添加的ATP通过NADPH氧化酶触发了保卫细胞中ROS的生成。ATP还诱导了保卫细胞中Ca2 +的流入和H +的流出。在atrbohD / F中,ATP诱导的离子通量被强烈抑制。在异源三聚体G蛋白α亚基的无效突变体中,ATP促进的气孔开放,细胞质ROS生成,Ca2+涌入和H+外排均被抑制。

这些结果表明,eATP促进的气孔开放可能涉及异三聚体G蛋白,ROS,胞质Ca2+和质膜H+ -ATPase。

Figure 6ATP Stimulates Ca2+ Influx and H+Efflux in Guard Cells of Arabidopsis thaliana (Ecotype col-0).

(A) Gadolinium chloride (50 μM) blocked ATP-promoted stomatal opening.

(B) and (C) show time course of Ca2+ flux in MES buffer without (B) or with (C) epidermis before and after 0.6 mM ATP treatment, respectively. The arrow marks time points of ATP treatment.

(D) The dose-dependence of ATP-promoted Ca2+ influx; data are the means ± SE (n = 30) of the peak Ca2+ influx value after ATP stimulation. The positive and negative values of ion flux represent ion influx and efflux, respectively.

(E) Sodium vanadate (100 μM) blocked ATP-promoted stomatal opening.

(F) and (G) show time courses of H+ flux in MES buffer without (F) or with (G) epidermis before and after 0.6 mM ATP treatment, respectively.

The arrow marks time points of ATP treatment. In (A) and (E), data are means ± SE (n = 6) for stomatal aperture. In all figures, ‘control’ means treatment with MES buffer only. (H) The dose-dependence of ATP-promoted H+ influx; data are the means ± SE (n = 30) of the peak H+ efflux value after ATP stimulation.

文章链接:https://www.cell.com/molecular-plant/fulltext/S1674-2052(14)60201-X?_returnURL