MP谢旗郭岩：Na+流为SOS调控植物耐盐新机制研究提供证据 | NMT植物耐盐创新科研平台
NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。
Mol Plant| 中科院遗传发育所谢旗团队揭示植物耐盐途径中关键因子作用的机制
研究使用平台：NMT植物耐盐创新科研平台
期刊：Molecular Plant
主题：Na+流为SOS调控植物耐盐新机制研究提供证据
标题：ESCRT-I component VPS23A sustains salt tolerance by strengthening the SOS module in Arabidopsis
影响因子：12.084
检测指标：Na+流速
检测样品：拟南芥根部分生区
Na+流实验处理方法：
12日龄的拟南芥在150 mM NaCl处理5h
Na+流实验测试液成份：
0.1 mM CaCl2, 0.1 mM KCl, 0.5 mM NaCl, and 0.3 mM MES，pH5.8
作者：谢旗、郭岩、于菲菲、娄丽娟
中文摘要（谷歌机翻）
含钠转运蛋白SOS1和调节蛋白SOS2和SOS3的盐分过度敏感（SOS）信号模块，众所周知是帮助植物抵抗盐分积累的中央盐分排泄系统。
在这里我们报告说，VPS23A是运输所需的内体分选复合物（ESCRT）的组成部分，在SOS模块赋予植物耐盐性的功能中起着至关重要的作用。VPS23A增强了SOS2 / SOS3复合体的相互作用。
在存在盐胁迫的情况下，VPS23A积极调节SOS2向质膜的重新分布过程，然后激活SOS1的反转运蛋白活性以减少植物细胞中的Na+积累。遗传证据表明，通过SOS2和SOS3的过表达实现的耐盐性取决于VPS23A。两者合计，我们的结果表明，VPS23A是SOS模块的关键调节因子，可影响SOS2在细胞膜上的亚细胞定位。
此外，膜结合的SOS2赋予拟南芥幼苗较强的耐盐性，揭示了SOS2分选对细胞膜发挥作用的重要性。
(D and E)Net Na+ fluxes in root tips. Non-invasive Micro-test Technology (NMT) was used for Na+ flux measurement in vivo. 10-day-old seedlings cultured in liquid ½MS medium were treated with 0 (D, upper panel) or 150 mM NaCl (D, lower panel) for 5 h, and then the continuous transient Na+ fluxes were recorded for about 6 min. Each point is the mean of four individual plants (D). Quantitative analysis of the means of net Na+ fluxes within the continuous period of 0–6 min as shown in (E).The values are means ± SD of three independent repeats. *P value <0.05.
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英文摘要
Salt-Overly-Sensitive (SOS) signaling module comprising the sodium-transport protein SOS1 and the regulatory proteins SOS2 and SOS3, is well known as the central salt excretion system that helps plants against salt accumulation.
Here we report that VPS23A, a component of the endosomal sorting complex required for transport (ESCRT), plays an essential role in the function of the SOS module to confer plant salt tolerance. VPS23A enhances the interaction of the SOS2/SOS3 complex.
In the presence of salt stress, VPS23A positively regulates the process of re-distribution of SOS2 to the plasma membrane and then activates the antiporter activity of SOS1 to reduce Na+ accumulation in plant cells. Genetic evidence demonstrated that salt tolerance achieved by the overexpression of SOS2 and SOS3 depended on VPS23A. Taken together, our results identified that VPS23A is a crucial regulator of the SOS module that affects the subcellular localization of SOS2 to the cell membrane.
Moreover, the strong salt tolerance of Arabidopsis seedlings conferred by a membrane-bound SOS2 revealed the significance of the action of SOS2 sorting to the cell membrane in achieving its function.
文章链接：https://doi.org/10.1016/j.molp.2020.05.010
2019版《NMT论文集》已出版
关键词：非损伤微测技术，Na+流速，盐胁迫
