ECOTOX ENVIRON SAFE ：中国海大丨Ca2+流可作为防污涂料性能评价指标（附NMT实验体系）
- 期刊：Ecotoxicology and Environmental Safety
- 标题：Synthesis and antifouling evaluation of indole derivatives
- 检测指标： Ca2+流速
- Ca2+流实验测试液成份：0.1mM KCl, 0.1mM CaCl2, 0.1mM MgCl2, 0.5mM NaCl, 0.3mM MES,0.2mM Na2SO4 and 0.1% sucrose, pH 7.8
Indole derivatives derived from the secondary metabolites of marine organisms possess the excellent antifouling property to inhibit the biofouling. These compounds and their analogues are simple in structure and have been proven to have low toxicity and bioaccumulation. Therefore, the active indole antifoulants are expected to replace the potentially toxic antifoulants which are widely used in current antifouling coatings.
Seven indole derivatives were synthesized via the Friedel-Crafts alkylation reaction and were characterized by IR spectra, 1H NMR, 13C NMR and elemental analysis. Inhibition experiments against marine algae and bacteria were conducted, and the partial inhibition rates of algae and bacteria were more than 90%. This outcome indicates that indole derivatives possess excellent properties suitable for use as targeting anti-fouling compound for algae and bacteria. Non-invasive Micro-test Technology (NMT) reveals that the Ca2+ efflux of Platymonas subcordiformis dramatically increased in the presence of indole derivatives, which is inferred to be the molecular mechanism for inhibiting the growth of marine algae.
The antifouling coatings containing indole derivatives were prepared and subjected to an antifouling test in a marine environment, and the results show that N-(1-H-5-bromo-indole-3-ylmethyl) benzamide and N-(1-H-2-phenyl-indole-3-ylmethyl) benzamide possess better antifouling performance compared to copper pyrithione (CuPT). According to these results, indole derivatives in this study might become novel and promising antifoulants.
通过Friedel-Crafts烷基化反应合成了7种吲哚衍生物，并通过IR光谱，1H NMR，13C NMR和元素分析进行了表征。对海藻和细菌进行了抑制实验，藻类和细菌的部分抑制率超过90％。该结果表明吲哚衍生物具有适合用作藻类和细菌的靶向防污化合物的优异性质。非侵入性微测试技术（NMT）揭示了在吲哚衍生物存在下，亚心形扁藻的Ca2+流出显着增加，这被推断为抑制海藻生长的分子机制。
制备含有吲哚衍生物的防污涂料并在海洋环境中进行防污试验，结果表明N-（1-H-5-溴 - 吲哚-3-基甲基）苯甲酰胺和N-（1-H-）与吡啶硫酮铜（CuPT）相比，2-苯基 - 吲哚-3-基甲基）苯甲酰胺具有更好的防污性能。根据这些结果，本研究中的吲哚衍生物可能成为新颖且有前景的防污剂。