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4月8日,某研究所将NMT技术应用于钙信号研究,测试样品为小麦,测试指标为Ca2+,在旭月研究院完成实验。| 5月9号,某研究院将NMT技术应用于逆境生理领域,测试样品为黄瓜幼苗,测试指标为NO3-、NH4+,在旭月研究院完成实验。| 6月2号,某研究院将NMT技术应用于逆境胁迫领域,测试样品为棉花苗,测试指标为Ca2+、H+、K+、Na+、IAA,在旭月研究院完成实验。| 6月5号,某研究院将NMT技术应用于植物逆境领域,测试样品为苜蓿,测试指标为K+,在旭月研究院完成实验。| 6月9号,某研究所将NMT技术应用于水稻逆境领域,测试样品为水稻,测试指标为Na+、Ca2+,在中国科学院植物研究所完成实验。| 6月11号,某研究院将NMT技术应用于植物抗逆领域,测试样品为酵母细胞,测试指标为IAA,在旭月研究院完成实验。| 6月16号,某高校将NMT技术应用于昆虫研究,测试样品为昆虫,测试指标为Ca2+、K+,在旭月研究院完成实验。| 6月19号,某研究院将NMT技术应用于植物抗逆领域,测试样品为拟南芥,测试指标为Ca2+,在旭月研究院完成实验。|

本文第一作者吴智敏文章解读

期刊:JOURNAL OF AGRICULTURALAND FOOD CHEMISTRY
主题:低氮提升油菜氮利用率新机制
标题:Low Nitrogen Enhances Nitrogen Use Efficiency by Triggering NO3– Uptake and Its Long-Distance Translocation
影响因子:3.412
检测指标:NO3-、H+
NO3-、H+流实验方法:油菜幼苗,15mM NO3-处理(CK)和1mM NO3-处理(LN),检测距离根尖600微米的点。
NO3-、H+流实验测试液成份:0.1 mM KNO3, 0.05 mM KCl, 0.15 mM NaCl, 0.05 mM CaCl2, 0.05 mM Mg CaCl2, and 0.3 mM MES ,pH 6.5
作者:湖南农业大学张振华、吴智敏
 

英文摘要

Nitrogen is essential for plant growth andcrop productivity; however, nitrogen use efficiency (NUE) decreases withincreasing N supply, resulting in a waste of resources. Molecular mechanismsunderlying low-nitrogen (LN)-mediated enhancement of NUE are not clear.

We used high-NUE Brassica napus genotype H(Xiangyou 15), low-NUE B. napus genotype L (814), and Arabidopsis mutant aux1to elucidate the mechanism underlying the changes in NUE under different ratesof N fertilizer application. NUE of B. napus increased under LN, which enhancedN uptake ability by regulating root system architecture and plasma membraneH+-ATPase activity; AUX1 was involved in this process.

Additionally, BnNRT1.5 was upregulated andBnNRT1.8 was downregulated under LN, whereby more N was transferred to theshoot through enhanced N transport. Observed changes in photosynthesis under LNwere associated with N assimilation efficiency. Our study provides new insightsinto the mechanisms of plant adaptation to the environment.
 

中文摘要(谷歌机翻译)

氮对植物生长和作物生产力至关重要;然而,随着氮供应的增加,氮利用效率(NUE)降低,导致资源浪费。低氮(LN)介导的NUE增强的分子机制尚不清楚。

我们使用高NUE甘蓝型油菜基因型H(香优15),低NUE B.napus基因型L(814)和拟南芥突变体aux1来阐明在不同氮肥施用量下NUE变化的机制。甘蓝型油菜NUE在LN下增加,通过调节根系结构和质膜H + -ATPase活性提高N吸收能力; AUX1参与了这个过程。

另外,BnNRT1.5被上调并且BnNRT1.8在LN下被下调,由此通过增强的N转运将更多的N转移到芽。观察到的LN下光合作用的变化与N同化效率有关。我们的研究为植物适应环境的机制提供了新的见解。

 

Gas exchange, chlorophyll content, and photosynthetic N use in two contrasting B. napus genotypes for N use efficiency under LN (1 mM NO3− N, black bars) or CK (15 mM NO3− N, white bars) conditions: (a) photosynthetic rate, (b) SPAD, (c) transpiration rate, and (d) photosynthetic N use efficiency (PNUE). The bar height represents the mean, and error bars indicate standard deviation (n = 6). Different letters on bars indicate significant differences based on Tukey’s HSD (p < 0.05). The statistical analysis was performed on different treatments within genotypes.

 

文章链接:https://pubs.acs.org/doi/10.1021/acs.jafc.9b02491

 

本课题组往期NMT文章:

  •   V-ATPase and V-PPase at the Tonoplast Affect NO3- Content in Brassica napus by Controlling Distribution of NO3- Between the Cytoplasm and Vacuole
  •   Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus
  •   NRT1.1-related NH4+ toxicity is associated with balance between NH4+ uptake and assimilation