NMT历史上的今天丨Traffic 植物营养 文章发表


2017年11月07日,首都师范大学印莉萍、谭松利用NMT在Traffic上发表了标题为TMD1 domain and CRAC motif determine the association and disassociation of MxIRT1 with detergent‐resistant membranes的研究成果。


  • 期刊:Traffic
  • 主题:TMD1域和CRAC基序决定MxIRT1与耐去污剂膜的缔合和解离
  • 标题:TMD1 domain and CRAC motif determine the association and disassociation of MxIRT1 with detergent‐resistant membranes
  • 影响因子:4.133
  • 检测指标:、Cd2+流速
  • 通讯作者:首都师范大学印莉萍、谭松


Iron is essential for most living organisms. The iron‐regulated transporter1 (IRT1) plays a major role in iron uptake in roots, and its trafficking from endoplasmic reticulum (ER) to plasma membrane (PM) is tightly coordinated with changes in iron environment.

However, studies on the IRT1 response are limited. Here, we report that Malus xiaojinesis IRT1 (MxIRT1) associates with detergent‐resistant membranes (DRMs, a biochemical counterpart of PM microdomains), whereas the PM microdomains are known platforms for signal transduction in the PM. Depending on the shift of MxIRT1 from microdomains to homogeneous regions in PM, MxIRT1‐mediated iron absorption is activated by the cholesterol recognition/interaction amino acid consensus (CRAC) motif of MxIRT1.

MxIRT1 initially associates with DRMs in ER via its transmembrane domain 1 (TMD1), and thus begins DRMs‐dependent intracellular trafficking. Subsequently, MxIRT1 is sequestered in COPII vesicles via the ER export signal sequence in MxIRT1.

These studies suggest that iron homeostasis is influenced by the CRAC motif and TMD1 domain due to their determination of MxIRT1‐DRMs association.






G) Average Cd2+flux in the different transgenic Arabidopsis root hairs in 7-minute incubation with 0.05 mM CdCl2 measured by Non-invasion Micro-test Technology. The bars represent the means standard errors of 3 independent analyses (n = 3). Significant differences were determined by Student’s t test. *P < 0.05, **P < 0.01