Plant J:港中大、山东农大丨水稻OsCBL10启动子天然变异影响种子萌发期的耐淹性(附NMT实验体系)
期刊:Plant journal
主题:水稻OsCBL10启动子天然变异影响种子萌发期的耐淹性
标题:Natural variation in the promoter of rice calcineurin B‐like protein10 (Os CBL 10) affects flooding tolerance during seed germination among rice subspecies
影响因子:5.775
检测指标:Ca2+流速
检测样品:水稻距胚芽鞘尖端不同距离(0、50、100、150、200、250、300、350、400、450、500和550μm)
Ca2+流速实验处理方法:水稻种子水淹胁迫
Ca2+流实验测试液成份:0.1 mM CaCl2、0.1 mM KCl、0.3 mM MES,pH 6.0
作者:香港中文大学张建华、山东农业大学刘鹰高、施璐
文章简介
2018年5月,香港中文大学张建华、山东农业大学刘鹰高教授课题组在Plant Journal杂志上发表题为“Natural variation in the promoter of rice Calcineurin B-like Protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies”的研究论文。
现代栽培水稻来源于旱稻的逐步演化,而这一演化过程的了解是揭示旱稻如何获得水淹抗性的关键,对于其它植物的耐水淹育种研究具有借鉴意义。
该研究通过分析水稻和旱稻的钙调磷酸酶B类蛋白(OsCBL10)的启动子区域发现两者存在差异。耐水淹的水稻品种都存在同样的T-Type类型启动子序列,此类型启动子会通过影响OsCBL10表达进而调节水淹胁迫下的Ca2+信号。
该项研究中,利用NMT技术检测了两个水稻栽培品种Low88和Up221胚芽鞘以及距离胚芽鞘尖端不同距离部位的Ca2+流速,结果显示两个品种直接的Ca2+流速存在差异。
结果表明:水淹胁迫下水稻胚芽鞘的Ca2+流速变化。
英文摘要
Rice (Oryza sativa L.) has two ecotypes, upland and lowland rice, that have been observed to show different tolerance levels under flooding stress.
In this study, two rice cultivars, upland (Up221, flooding‐intolerant) and lowland (Low88, flooding‐tolerant), were initially used to study their molecular mechanisms in response to flooding germination. We observed that variations in the OsCBL10 promoter sequences in these two cultivars might contribute to this divergence in flooding tolerance. Further analysis using another eight rice cultivars revealed that the OsCBL10 promoter could be classified as either a flooding‐tolerant type (T‐type) or a flooding‐intolerant type (I‐type).
The OsCBL10 T‐type promoter only existed in japonica lowland cultivars, whereas the OsCBL10 I‐type promoter existed in japonica upland, indica upland and indica lowland cultivars. Flooding‐tolerant rice cultivars containing the OsCBL10 T‐type promoter have shown lower Ca2+ flow and higher α‐amylase activities in comparison to those in flooding‐intolerant cultivars.
Furthermore, the OsCBL10 overexpression lines were sensitive to both flooding and hypoxic treatments during rice germination with enhanced Ca2+ flow in comparison to wild‐type. Subsequent findings also indicate that OsCBL10 may affect OsCIPK15 protein abundance and its downstream pathways.
In summary, our results suggest that the adaptation to flooding stress during rice germination is associated with two different OsCBL10 promoters, which in turn affect OsCBL10 expression in different cultivars and negatively affect OsCIPK15 protein accumulation and its downstream cascade.
中文摘要(谷歌机翻译)
水稻(Oryza sativa L.)有两种生态型,旱稻和低陆稻,在洪水胁迫下它们表现出不同的耐受水平。
在这项研究中,最初使用两个水稻品种,高地(Up221,耐水)和低地(Low88,耐水)来研究其对洪水萌发的分子机制。我们观察到,在这两个品种中,OsCBL10启动子序列的变异可能导致这种泛洪耐受性的差异。使用另外八个水稻品种的进一步分析表明,OsCBL10启动子可以分为耐水型(T型)或耐水型(I型)。
OsCBL10 T型启动子仅存在于粳稻低地品种中,而OsCBL10 I型启动子则存在于粳稻高地,in稻高地和low稻低地品种中。与耐水不耐旱品种相比,含有OsCBL10 T型启动子的耐水耐旱水稻品种显示出较低的Ca2+流量和较高的α-淀粉酶活性。
此外,与野生型相比,OsCBL10过表达品系对水稻发芽期间的水淹和低氧处理均敏感,且Ca2+流量增加。随后的发现还表明,OsCBL10可能会影响OsCIPK15蛋白的丰度及其下游途径。
总而言之,我们的结果表明,水稻发芽过程中对洪水胁迫的适应与两个不同的OsCBL10启动子有关,这反过来影响了OsCBL10在不同品种中的表达,并对OsCIPK15蛋白的积累及其下游级联反应产生了负面影响。
文章链接:https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13881