NMT历史上的今天丨Chemosphere：TiO2提升黄孢原毛平革菌镉耐受力的生理证据

---

NMT历史上的今天

2014年12月24日，湖南大学曾光明、陈桂秋、谭琼用NMT在Chemosphere上发表了标题为Physiological fluxes and antioxidative enzymes activities of immobilized Phanerochaete chrysosporium loaded with TiO2 nanoparticles after exposure to toxic pollutants in solution的研究成果。

 

• 期刊：Chemosphere
• 主题：TiO₂提升黄孢原毛平革菌镉耐受力的生理证据
• 标题：Physiological fluxes and antioxidative enzymes activities of immobilized Phanerochaete chrysosporium loaded with TiO₂ nanoparticles after exposure to toxic pollutants in solution
• 影响因子：4.208
• 检测指标：H⁺、O₂、Cd²⁺流速
• 通讯作者：湖南大学曾光明、陈桂秋、谭琼

英文摘要

Immobilized Phanerochaete chrysosporium loaded with TiO2 nanoparticles (PTNs) are novel high-value bioremediation materials for adsorbing cadmium and for degrading 2,4-dichlorophenol (2,4-DCP). The real-time changes in H⁺ and O₂ fluxes were measured using the noninvasive microtest technique (NMT).

The H⁺ influx increased after the addition of 2,4-DCP, and shifted to efflux following the addition of Cd²⁺. The O₂ flux decreased after the addition of both 2,4-DCP and Cd²⁺. A larger Cd²⁺ flux was immediately observed after exposure to 0.5 mM Cd²⁺ (−351.25 pmol cm−2 s−1) than to 0.1 mM Cd²⁺ (−107.47 pmol cm−2 s−1). The removal of Cd²⁺ by the PTNs increased more after treatment with the 0.5 mM exposure solution (27.6 mg g−1) than with the 0.1 mM exposure solution (3.49 mg g−1).

The enzyme activities were analyzed to review the antioxidative defense system of PTNs in a solution containing various concentrations of Cd²⁺. The activities of the coenzyme nicotinamide adenine dinucleotide (NADH) oxidase as well as the enzyme catalase (CAT) plateaued at 6.5 U g−1 FW and 9.7 U g−1 FW, respectively, after exposure to 0.25 mM Cd2+. The activity of superoxide dismutase (SOD) increased gradually in solutions containing 0.1–0.6 mM Cd2+, and eventually reached a maximum (68.86 U g−1 FW).

These results illustrate how the antioxidative defense system and the physiological fluxes of PTNs respond to the stress caused by toxic pollutants.

中文摘要（谷歌机翻）

负载有TiO2纳米粒子（PTNs）的固定化Phanerochaete chsssporium是用于吸附镉和降解2,4-二氯苯酚（2,4-DCP）的新型高价值生物修复材料。使用非损伤微测技术（NMT）测量H⁺和O₂通量的实时变化。

加入2,4-DCP后，H⁺流入量增加，而加入Cd²⁺后，H⁺流入量移出。同时添加2,4-DCP和Cd²⁺后，O2通量下降。暴露于0.5 mM Cd²⁺（-351.25 pmol cm^-2 s^-1）后立即观察到更大的Cd²⁺通量，而不是暴露于0.1 mM Cd²⁺（-107.47 pmol cm^-2 2 s^-1）。用0.5 mM暴露溶液（27.6 mg g-1）处理后，PTN去除Cd²⁺的增加比使用0.1 mM暴露溶液（3.49 mg g-1）处理的增加更多。

分析了酶的活性，以审查包含各种浓度的Cd²⁺的溶液中PTN的抗氧化防御系统。暴露于0.25 mM Cd²⁺后，辅酶烟酰胺腺嘌呤二核苷酸（NADH）氧化酶和过氧化氢酶（CAT）的活性分别稳定在6.5 U g-1 FW和9.7 U g-1 FW上。超氧化物歧化酶（SOD）的活性在含0.1–0.6 mM Cd²⁺的溶液中逐渐增加，并最终达到最大值（68.86 U g-1 FW）。

这些结果说明了PTN的抗氧化防御系统和生理通量如何响应有毒污染物引起的压力。

Fig. 2. (A) Real-time Cd²⁺ flux of PTNs exposed to 0.1 mM Cd(NO3)2 and 10 mg L

文章链接：https://www.sciencedirect.com/science/article/pii/S0045653515000326?via%3Dihub