NMT历史上的今天丨Plant Physiol:TaNAC2-5A调控硝酸盐提升小麦产量


NMT历史上的今天

2015年12月27日,中科院遗传发育所童依平、何雪用NMT在Plant Physiology上发表了标题为The Nitrate-Inducible NAC Transcription Factor TaNAC2-5A Controls Nitrate Response and Increases Wheat Yield的研究成果。

 

  • 期刊:Plant Physiology
  • 主题:TaNAC2-5A控制硝酸盐反应提高小麦产量
  • 标题:The Nitrate-Inducible NAC Transcription Factor TaNAC2-5A Controls Nitrate Response and Increases Wheat Yield
  • 影响因子:7.394
  • 检测指标:NO3-流速
  • 通讯作者:中科院遗传发育所童依平、何雪

英文摘要

Nitrate is a major nitrogen resource for cereal crops; thus, understanding nitrate signaling in cereal crops is valuable for engineering crops with improved nitrogen use efficiency. Although several regulators have been identified in nitrate sensing and signaling in Arabidopsis (Arabidopsis thaliana), the equivalent information in cereals is missing.

Here, we isolated a nitrate-inducible and cereal-specific NAM, ATAF, and CUC (NAC) transcription factor, TaNAC2-5A, from wheat (Triticum aestivum). A chromatin immunoprecipitation assay showed that TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase. Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate and, hence, increased the root’s ability to acquire nitrogen. Furthermore, we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts and allocated more nitrogen in grains in a field experiment.

These results suggest that TaNAC2-5A is involved in nitrate signaling and show that it is an exciting gene resource for breeding crops with more efficient use of fertilizer.

中文摘要(谷歌机翻)

硝酸盐是谷物作物的主要氮资源。因此,了解谷类作物中的硝酸盐信号对于提高氮素利用效率的工程作物非常有价值。尽管已经在拟南芥(Arabidopsis thaliana)的硝酸盐感测和信号传导中确定了几种调节剂,但谷物中的等效信息却缺失了。

在这里,我们从小麦(Triticum aestivum)中分离出了硝酸盐诱导型和谷类特异性NAM,ATAF和CUC(NAC)转录因子TaNAC2-5A。染色质免疫沉淀分析表明,TaNAC2-5A可以直接结合到编码硝酸盐转运蛋白和谷氨酰胺合成酶的基因的启动子区域。TaNAC2-5A在小麦中的过表达增强了根系的生长和硝酸盐的流入速率,因此增加了根系获得氮的能力。此外,我们发现在田间试验中,过表达TaNAC2-5A的转基因小麦品系具有较高的籽粒产量和较高的氮素在地上部分的积累,并在籽粒中分配了更多的氮。

这些结果表明,TaNAC2-5A参与硝酸盐信号传导,并表明它是利用更有效地使用肥料来育种作物的令人兴奋的基因资源。

D and E, Nitrate influx rates at the root tip surface (D) and expression levels of nitrate transporters in whole seedlings (E). Seedlings were grown for 4 d on solid medium that contained 6 mM nitrate. The roots were used to measure nitrate flux rate in the measuring solution that contained 1 mM nitrate. Data are means 6 SE for six plants. Whole seedlings were used to analyze the expression of nitrate trans-porters. The relative expression levels were normalized to the expression of AtACTIN2 (AtACT2). Data are means 6 SE of three replicates. Asterisks indicate that the difference between the means of the wild type and the transgenic lines was significant at the P , 0.05 (*) and P , 0.01 (**) levels.

文章链接:http://www.plantphysiol.org/content/169/3/1991

 

 

中关村NMT联盟全国测试服务网络测试服务信息

4月8日,某研究所将NMT技术应用于钙信号研究,测试样品为小麦,测试指标为Ca2+,在旭月研究院完成实验。| 5月9号,某研究院将NMT技术应用于逆境生理领域,测试样品为黄瓜幼苗,测试指标为NO3-、NH4+,在旭月研究院完成实验。| 6月2号,某研究院将NMT技术应用于逆境胁迫领域,测试样品为棉花苗,测试指标为Ca2+、H+、K+、Na+、IAA,在旭月研究院完成实验。| 6月5号,某研究院将NMT技术应用于植物逆境领域,测试样品为苜蓿,测试指标为K+,在旭月研究院完成实验。| 6月9号,某研究所将NMT技术应用于水稻逆境领域,测试样品为水稻,测试指标为Na+、Ca2+,在中国科学院植物研究所完成实验。| 6月11号,某研究院将NMT技术应用于植物抗逆领域,测试样品为酵母细胞,测试指标为IAA,在旭月研究院完成实验。| 6月16号,某高校将NMT技术应用于昆虫研究,测试样品为昆虫,测试指标为Ca2+、K+,在旭月研究院完成实验。| 6月19号,某研究院将NMT技术应用于植物抗逆领域,测试样品为拟南芥,测试指标为Ca2+,在旭月研究院完成实验。|