Arch of Biochem and Biophys : NMT揭示近视小鼠睫状肌K+稳态被破坏致微环境紊乱
NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。
中国水科院黄海水产研究所叶乃好团队在气候变化条件下藻类演变趋势及机制研究领域获突破性进展
研究使用平台：NMT在体组织研究创新科研平台
期刊：Archives of Biochemistry and Biophysics
主题：NMT揭示近视小鼠睫状肌K+稳态被破坏致微环境紊乱
标题：Disrupted potassium ion homeostasis in ciliary muscle in negative lensinduced myopia in Guinea pigs
影响因子：3.559
检测指标：
K+流速
检测样品：豚鼠眼部睫状肌
K+流实验处理方法：
对4周对照组和4周负性晶状体近视组豚鼠进行麻醉后摘除角膜，暴露睫状肌
K+流实验测试液成份：
122.18 mM NaCl,5.1 mMKCl, 1.05 mM CaCl2·2H2O,0.98 mM MgCl2·6H2O, 2.96 mM Na2HPO4,25 mM NaHCO3, 5.11 mM D-Glucose
作者：山东中医药大学眼科研究所毕宏生、郭大东、吴珊珊
中文摘要（谷歌机翻）
近视是可预防或可治疗的视力障碍的主要原因，由于其在世界范围内的患病率越来越高，它已成为主要的公共卫生问题。目前，已经证实近视的发展与适应性疾病有关。
作为调节的主要因素，睫状肌收缩/松弛可以调节晶状体的生理状态，并且在近视的发展中起关键作用。为了研究近视与睫状肌的关系，将豚鼠随机分为正常对照组（NC）组和负晶状体诱发性近视（LIM）组，并将每组动物进一步随机分为2周（n = 18）和4周（n = 21）的亚组，分别根据2周和4周的近视诱导时间而定。
在本研究中，LIM组动物的右眼用−6.0 D透镜遮盖引起近视。接下来，我们进行了苏木精和曙红（H＆E）染色以观察睫状肌的病理变化，确定三磷酸腺苷（ATP）和乳酸（LA）的含量，并测量Na+/ K+ -ATPase在睫状肌中的表达和活性NC和LIM组的肌肉。
此外，我们还分析了4周NC和LIM豚鼠睫状肌中的钾离子（K+）通量。结果，我们发现LIM豚鼠的睫状肌排列被破坏，溶解或混乱。LIM豚鼠睫状肌中ATP含量降低，而LA含量升高。对LIM豚鼠睫状肌中K+通量的监测表明，近视触发了K+内流。此外，我们还注意到，来自LIM豚鼠的Na+ / K+ -ATPase（Atp1a1）在mRNA和蛋白质水平上的表达均降低，在睫状肌中的活性降低。
总体而言，我们的结果将有助于了解与晶状体引起的近视眼中抑制性Na+ / K+ -ATPase相关的机制，从而导致LIM豚鼠睫状肌微环境紊乱，为有希望的辅助治疗方法铺平了道路。在临床实践中治疗近视。
Determination of potassium ion fluxes in ciliary muscles of guinea pigs in 4-week NC and 4-week LIM subgroups. After myopic induction for 4 weeks, the guinea pigs were anesthetized and the ciliary muscles were exposed. Noninvasive Micro-test Technology (NMT) was applied to measure the real-time K+ fluxes in ci iary muscle tissues in NC and LIM guinea pigs (A). Histogram analysis (B) indicated that the trend of K+ flux measured in NC guinea pigs was in a stable flux status around the baseline (128.20 ± 178.42 pmol cm−2·s−1), while the K+ flux measured in LIM guinea pigs was in an influx status (−476.96 ± 243.42 pmol cm−2·s−1).
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英文摘要
Myopia is a main cause of preventable or treatable visual impairment, it has become a major public health issue due to its increasingly high prevalence worldwide. Currently, it is confirmed that the development of myopia is associated with the disorders of accommodation.
As a dominant factor for accommodation, ciliary muscle contraction/relaxation can regulate the physiological state of the lens and play a crucial role in the development of myopia. To investigate the relationship between myopia and ciliary muscle, the guinea pigs were randomly divided into a normal control (NC) group and a negative lens-induced myopia (LIM) group, and the animals in each group were further randomly assigned into 2-week (n = 18) and 4-week (n = 21) subgroups in accordance with the duration of myopic induction of 2 and 4 weeks, respectively.
In the present study, right eyes of the animals in LIM group were covered with −6.0 D lenses to induce myopia. Next, we performed the haematoxylin and eosin (H&E) staining to observe the pathological change of ciliary muscle, determined the contents of adenosine triphosphate (ATP) and lactate acid (LA), and measured the Na+/ K+-ATPase expression and activity in ciliary muscles in both NC and LIM groups.
Moreover, we also analyzed the potassium ion (K+) flux in ciliary muscles from 4-week NC and LIM guinea pigs. As a result, we found that the arrangements of ciliary muscles in LIM guinea pigs were broken, dissolved or disorganized; the content of ATP decreased, whereas the content of LA increased in ciliary muscles from LIM guinea pigs. Monitoring of K+ flux in ciliary muscles from LIM guinea pigs demonstrated myopia-triggered K+ influx. Moreover, we also noted a decreased expression of Na+/ K+-ATPase (Atp1a1) at both mRNA and protein levels and reduced activity in ciliary muscles from LIM guinea pigs.
文章链接：https://doi.org/10.1016/j.abb.2020.108403
2019版《NMT论文集》已出版
关键词：非损伤微测技术，K+流速，睫状肌
